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files raw  (ATCC)


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    ATCC files raw
    Files Raw, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 86 article reviews
    files raw - by Bioz Stars, 2026-03
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    Base editing in EcN and cyanobacterium Synechococcus elongatus PCC7942 with XSTART . A , sequencing results of argH in EcN edited by XSTART with pBeSpRY-argH. B , phenotypical evaluation of the argH -inactivated EcN. C , customization of XSTART for cyanobacteria via replacing the dCas9–AID module on pSY plasmid with the dSpRY–AID module. D , sequencing results of nblA in S . elongatus PCC7942. E , phenotypical evaluation of the nblA -inactivated S . elongatus PCC7942 in nitrogen-rich and depletion conditions. The edited loci are indicated by red arrows and highlighted in red . Three independent isolates of the edited strain were randomly chosen for phenotypical evaluation, and all tested strains were plasmid cured. AID, activation-induced cytidine deaminas; EcN, E . coli Nissle 1917.

    Journal: The Journal of Biological Chemistry

    Article Title: One-for-all gene inactivation via PAM-independent base editing in bacteria

    doi: 10.1016/j.jbc.2024.108113

    Figure Lengend Snippet: Base editing in EcN and cyanobacterium Synechococcus elongatus PCC7942 with XSTART . A , sequencing results of argH in EcN edited by XSTART with pBeSpRY-argH. B , phenotypical evaluation of the argH -inactivated EcN. C , customization of XSTART for cyanobacteria via replacing the dCas9–AID module on pSY plasmid with the dSpRY–AID module. D , sequencing results of nblA in S . elongatus PCC7942. E , phenotypical evaluation of the nblA -inactivated S . elongatus PCC7942 in nitrogen-rich and depletion conditions. The edited loci are indicated by red arrows and highlighted in red . Three independent isolates of the edited strain were randomly chosen for phenotypical evaluation, and all tested strains were plasmid cured. AID, activation-induced cytidine deaminas; EcN, E . coli Nissle 1917.

    Article Snippet: E . coli MG1655 (CGSC#6300) strain, EcN strain, and cyanobacteria S . elongatus PCC7942 (American Type Culture Collection 33912) strain were used to test the feasibility of base-editing system.

    Techniques: Sequencing, Plasmid Preparation, Activation Assay